2024-03-24 at 7:34 PM UTC
You know, it's funny when it rains it pours
They got money for wars, but can't feed the poor!!
Said it ain't no hope for the youth and the truth is…
It ain't no hope for the future! ❤️🎵
2024-03-24 at 9:15 PM UTC
I'm gettin' mine, baby
Gotta let these niggas know, yeah
Gettin' right, babe
You should go and get your own
I'm gettin' mine, baby
Gotta let these niggas know, yeah
Gettin' right, babe
You should go and get your own ! ❤️🎵
2024-03-24 at 10:05 PM UTC
Instigator
Naturally Camouflaged
[the staring tame crusher]
People always jockin my style
2024-03-24 at 10:18 PM UTC
dey aint got no money to fee da poor,
coz dey have to feed dem lazy negroes.
2024-03-24 at 10:20 PM UTC
They figured out a way to pull DNA from footprints in the snow.
2024-03-25 at 1:19 AM UTC
Six samples from fresh (<48h old) fox tracks were collected in Jämtland County, Sweden, during the winter in 2006. Samples were taken from different tracks, so as to minimise the risk of sampling the same individual twice. Each imprint was carefully excavated from the snow using sterile equipment and put into 50ml Falcon tubes. Three additional samples were taken from undisturbed snow, for use as negative controls. Due to the possibility of low DNA yields from footprints, and ensuing risk of contamination, the DNA extractions were done in a dedicated ancient DNA facility in Madrid, Spain. The samples were concentrated using 15 ml Amicon filters (Millipore Massachusetts, USA) and extracted from the resulting filtrate following the pro tocol by Yang et al. (1998), as modified by Svensson et al. (2007). An approximately 150 base pair (bp) fragment of the mitochondrial control region was amplified using primers Pex3F and H3R (Dalén et al. 2007). Resulting PCR products were sequenced using the Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, California, USA) and analysed on an Applied Biosystems 3730xl sequencer following the manufacturer’s instructions. Previously published Arctic Fox (Alopex lagopus) and Red Fox (Vulpes vulpes) mitochondrial DNA sequences covering the length of the 150-bp amplifid fragment were collected from GenBank (accession numbers AF338791-AF338792, AF338794-AF338796, AF338 798-AF338801, AY321120-AY321148) and aligned by eye. A Maximum Likelihood (ML) phylogenetic analysis was performed using the program PAUP v4.10b (Swofford 1999). An HKY model of nucleotide substitution was assumed, so as to allow for different rates of transitions and transversions along the sequences. Starting trees were generated by neighbor-joining (NJ), from which subsitution parameters were estimated and fixed. Heuristic searches were then per formed using the estimated substitution parameters and SPR branch swapping. Substitution parameters were then re-estimated from the most likely trees and the analysis was repeated. Figure 1 shows the resulting ML tree describing the relationship of the sequence amplified from the footprint with Arctic and Red foxes.The two clades are separated by bootstrap (100 full heuristic runs, with starting trees generated by NJ and NNI branch swapping) support values of 96%.
Results:
Mitochondrial DNA was recovered from one of the six footprints from which snow was collected. While it has been shown recently that DNA can be recovered from faecal and hair samples collected along animal tracks (Ulizio et al. 2006), this is the first report of DNA being recovered directly from the tracks themselves. Identification of species from tracks presents an important resource for conservation and management of rare species. In this case, both Arctic and Red foxes inhabit the Swedish mountain tundra, and the footprint could thus have derived from either of the two species. However, the resulting mtDNA sequence showed that the footprint originated from an Arctic Fox and was identical to one of the three mtDNA haplotypes that exist today in Sweden (Dalénet al. 2005).
2024-03-25 at 1:37 AM UTC
Your candle burned out long before
But your legend never will ❤️🎵 🎹
2024-03-25 at 1:40 AM UTC
They crawled out of the woodwork
And whispered into your brain
They put you on a treadmill
And made you change your name ! ❤️🎵🎹
2024-03-25 at 1:44 AM UTC
The following users say it would be alright if the author of this
post didn't die in a fire!
2024-03-25 at 1:44 AM UTC
Hollywood created a superstar 🌟
And pain was the price you paid
Even when you died
Oh, the press still hounded you
All the papers had to say
Was that Marilyn was found… in the nude!❤️🎵🎹
2024-03-25 at 2:52 AM UTC
I like candyrein's posts on our forum
The following users say it would be alright if the author of this
post didn't die in a fire!
The following users say it would be alright if the author of this post didn't die in a fire!
