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  1. CandyRein Black Hole
    😭😂
  2. CandyRein Black Hole


    ❤️
  3. CandyRein Black Hole
    You know, it's funny when it rains it pours

    They got money for wars, but can't feed the poor!!

    Said it ain't no hope for the youth and the truth is…

    It ain't no hope for the future! ❤️🎵
  4. CandyRein Black Hole


    ❤️
  5. CandyRein Black Hole
    I'm gettin' mine, baby
    Gotta let these niggas know, yeah

    Gettin' right, babe
    You should go and get your own

    I'm gettin' mine, baby
    Gotta let these niggas know, yeah

    Gettin' right, babe
    You should go and get your own ! ❤️🎵
  6. Instigator Naturally Camouflaged [the staring tame crusher]
    People always jockin my style
  7. dey aint got no money to fee da poor,

    coz dey have to feed dem lazy negroes.
  8. Bradley Florida Man
    They figured out a way to pull DNA from footprints in the snow.
  9. Six samples from fresh (<48h old) fox tracks were collected in Jämtland County, Sweden, during the winter in 2006. Samples were taken from different tracks, so as to minimise the risk of sampling the same individual twice. Each imprint was carefully excavated from the snow using sterile equipment and put into 50ml Falcon tubes. Three additional samples were taken from undisturbed snow, for use as negative controls. Due to the possibility of low DNA yields from footprints, and ensuing risk of contamination, the DNA extractions were done in a dedicated ancient DNA facility in Madrid, Spain. The samples were concentrated using 15 ml Amicon filters (Millipore Massachusetts, USA) and extracted from the resulting filtrate following the pro tocol by Yang et al. (1998), as modified by Svensson et al. (2007). An approximately 150 base pair (bp) fragment of the mitochondrial control region was amplified using primers Pex3F and H3R (Dalén et al. 2007). Resulting PCR products were sequenced using the Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, California, USA) and analysed on an Applied Biosystems 3730xl sequencer following the manufacturer’s instructions. Previously published Arctic Fox (Alopex lagopus) and Red Fox (Vulpes vulpes) mitochondrial DNA sequences covering the length of the 150-bp amplifid fragment were collected from GenBank (accession numbers AF338791-AF338792, AF338794-AF338796, AF338 798-AF338801, AY321120-AY321148) and aligned by eye. A Maximum Likelihood (ML) phylogenetic analysis was performed using the program PAUP v4.10b (Swofford 1999). An HKY model of nucleotide substitution was assumed, so as to allow for different rates of transitions and transversions along the sequences. Starting trees were generated by neighbor-joining (NJ), from which subsitution parameters were estimated and fixed. Heuristic searches were then per formed using the estimated substitution parameters and SPR branch swapping. Substitution parameters were then re-estimated from the most likely trees and the analysis was repeated. Figure 1 shows the resulting ML tree describing the relationship of the sequence amplified from the footprint with Arctic and Red foxes.The two clades are separated by bootstrap (100 full heuristic runs, with starting trees generated by NJ and NNI branch swapping) support values of 96%.

    Results:

    Mitochondrial DNA was recovered from one of the six footprints from which snow was collected. While it has been shown recently that DNA can be recovered from faecal and hair samples collected along animal tracks (Ulizio et al. 2006), this is the first report of DNA being recovered directly from the tracks themselves. Identification of species from tracks presents an important resource for conservation and management of rare species. In this case, both Arctic and Red foxes inhabit the Swedish mountain tundra, and the footprint could thus have derived from either of the two species. However, the resulting mtDNA sequence showed that the footprint originated from an Arctic Fox and was identical to one of the three mtDNA haplotypes that exist today in Sweden (Dalénet al. 2005).
  10. CandyRein Black Hole


    ❤️
  11. CandyRein Black Hole
    Your candle burned out long before
    But your legend never will ❤️🎵 🎹
  12. CandyRein Black Hole



    ❤️
  13. infinityshock Black Hole
    Originally posted by ⠀⠀⠀⠀⠀⠀ Six samples from fresh (<48h old) fox tracks were collected in Jämtland County, Sweden, during the winter in 2006. Samples were taken from different tracks, so as to minimise the risk of sampling the same individual twice. Each imprint was carefully excavated from the snow using sterile equipment and put into 50ml Falcon tubes. Three additional samples were taken from undisturbed snow, for use as negative controls. Due to the possibility of low DNA yields from footprints, and ensuing risk of contamination, the DNA extractions were done in a dedicated ancient DNA facility in Madrid, Spain. The samples were concentrated using 15 ml Amicon filters (Millipore Massachusetts, USA) and extracted from the resulting filtrate following the pro tocol by Yang et al. (1998), as modified by Svensson et al. (2007). An approximately 150 base pair (bp) fragment of the mitochondrial control region was amplified using primers Pex3F and H3R (Dalén et al. 2007). Resulting PCR products were sequenced using the Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, California, USA) and analysed on an Applied Biosystems 3730xl sequencer following the manufacturer’s instructions. Previously published Arctic Fox (Alopex lagopus) and Red Fox (Vulpes vulpes) mitochondrial DNA sequences covering the length of the 150-bp amplifid fragment were collected from GenBank (accession numbers AF338791-AF338792, AF338794-AF338796, AF338 798-AF338801, AY321120-AY321148) and aligned by eye. A Maximum Likelihood (ML) phylogenetic analysis was performed using the program PAUP v4.10b (Swofford 1999). An HKY model of nucleotide substitution was assumed, so as to allow for different rates of transitions and transversions along the sequences. Starting trees were generated by neighbor-joining (NJ), from which subsitution parameters were estimated and fixed. Heuristic searches were then per formed using the estimated substitution parameters and SPR branch swapping. Substitution parameters were then re-estimated from the most likely trees and the analysis was repeated. Figure 1 shows the resulting ML tree describing the relationship of the sequence amplified from the footprint with Arctic and Red foxes.The two clades are separated by bootstrap (100 full heuristic runs, with starting trees generated by NJ and NNI branch swapping) support values of 96%.

    Results:

    Mitochondrial DNA was recovered from one of the six footprints from which snow was collected. While it has been shown recently that DNA can be recovered from faecal and hair samples collected along animal tracks (Ulizio et al. 2006), this is the first report of DNA being recovered directly from the tracks themselves. Identification of species from tracks presents an important resource for conservation and management of rare species. In this case, both Arctic and Red foxes inhabit the Swedish mountain tundra, and the footprint could thus have derived from either of the two species. However, the resulting mtDNA sequence showed that the footprint originated from an Arctic Fox and was identical to one of the three mtDNA haplotypes that exist today in Sweden (Dalénet al. 2005).

    or they could have just left sticky boards around bait stations and not had to make such an elaborate ordeal out of it.

    fucking scientists think theyre so smart
  14. CandyRein Black Hole


    ❤️
  15. CandyRein Black Hole
    They crawled out of the woodwork
    And whispered into your brain

    They put you on a treadmill
    And made you change your name ! ❤️🎵🎹
  16. ner vegas African Astronaut
    The following users say it would be alright if the author of this post didn't die in a fire!
  17. CandyRein Black Hole
    Hollywood created a superstar 🌟
    And pain was the price you paid

    Even when you died
    Oh, the press still hounded you

    All the papers had to say
    Was that Marilyn was found… in the nude!❤️🎵🎹
  18. Bradley Florida Man
    I like candyrein's posts on our forum
    The following users say it would be alright if the author of this post didn't die in a fire!
  19. CandyRein Black Hole
    Very mutual
  20. CandyRein Black Hole
    420 post
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