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Project Euryphaessa - Stage One
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2015-10-28 at 11:14 PM UTCIn Greek mythology, Euryphaessa is a Titan Goddess who, among other functions, serves as deity over the moon and vision. Her power comes from the Greek - none too dissimilar to the Victorian - belief that vision was the result of the eyes emitting rays of light that acted as a sort of radar. The moon, being the primary light source at night, was thus seen as a great eye in the sky of the Goddess Euryphaessa who could see everything in the night.
Project: Euryphaessa has been an on-going series of experiments I've been conducting and reporting elsewhere, but this is my "hello" to you. The idea is to enhance and even add to the available senses based on prior scientific research.
Biological night vision is the enhancement of your own eyes rather than using an electronic/chemically powered device to intensify available light. In this case, the basis of the tests was the SFM experiment as seen here:
http://scienceforthemasses.org/2015/...ng-chlorin-e6/
The PDF featured similarly breaks down the process but I'll hold your hand through the process to make this more accessible to you.
First, you need Chlorin E6, DMSO, human insulin, and saline solution.
Chlorin E6 is the light intensifier. DMSO dissolves and allows the Ce6 to more readily absorb into the eye. Insulin also aids in absorption but more importantly it slows transport of ABCG2 which increases Ce6 usage. Saline solution is simply a buffer to reach proper concentration.
Being able to measure properly is important. Failing lab grade supplies, you can convert the measurements to ml and use a common measuring spoon set to ensure you use the proper amount of each item. There is room for error. If you are barely over or under on any given thing, the risk is less healthwise and more efficiency of the night vision. You can also use existing droppers in lieu of a micropipette. 50ul is basically "one drop". Finally, sonication is mentioned as the mixing method. You can shake the combination vigorously but one thing you need to know is that you don't want to do this in a container which can catch particles. This was a particular headache I suffered when the Ce6 stuck to surfaces and failed to mix and dissolve initially. Make sure it's a tiny container with smooth surfaces!
So read the PDF. Get the items you need (sources below). Measure out each reagent in its proper amount either via small graduated cylinders or just use the spoons after conversion. Pour into mixing container - preferably something with tiny dropper drop so you don't need to transfer - using a funnel to ensure there is no spillage. Shake vigorously for a minute and place into a refrigerator immediately. Usage instructions forthcoming.
But first, let's talk reagent handling. Saline solution is straight forward. Insulin you should be careful with but note that it does not absorb through the skin readily - at least not without DMSO. It also needs to be kept refrigerated. DMSO absorbs easy as shit and you NEED to take care that it doesn't get on you because it will take anything else with it including potentially dangerous molecules. Finally, Ce6 needs to be kept fairly cold - in the freezer if kept in powdered form.
Usage requires some knowledge and care, you can't just drop and go. Dosage involves three drops per eye, or three 50ul drops. Use one hand to hold your eyelids open so you can't blink the solution out and apply one drop to the white on the inside of the pupil. After the drop, allow time for absorption and quickly use the same hand used to drop to put pressure just below your tear duct so solution does not escape that way. Repeat until you have absorbed three drops per eye.
At this point, your eyes will gradually increase in light sensitivity for the next 1.5-2 hours until reaching peak performance. From here you will more slowly degrade for roughly 6 hours until you return to normal vision. There are no after effects - once the effect is gone it's gone. Sensitivity will be such that bright lights will cause you problems and I advise you to work out a solution ahead of time to prepare for this. For certain endeavors, I recommend a black nylon shade that can be pulled over the eyes for bright areas or lights pointed toward you. There is risk of staring into bright lights but don't worry - you're not going to be staring into bright lights. It's too repulsive and you will instinctively look away preventing it from being a problem.
Think of the effect as your eyes adjusting to the night. Only with the drops, you adjust another order of magnitude that much more.
A lot of research has gone into this and I am probably taking a lot for granted and leaving things out on preparing and applying the Ce6 solution. If anyone has questions on this, please let me know.
REAGENT ACQUISITION:
Where do you get your shit? Well it's not too bad.
Saline solution can be found anywhere. Might as well bundle it with your DMSO though, because the best source for that is probably Amazon. Get yourself some 99% DMSO and standard saline flushing solution.
Ce6 can be had at Medkoo bioscience. You will need to email them for the order but it is painless and they will ship ASAP. International orders are also possible so good news to those outside of the US.
This leaves insulin. I acquired mine from a diabetic locally, no questions asked. As I understand it, you can obtain some brands without a prescription but I don't know about international acquisition of this. In theory the formula should work without it if that's what it comes down to, but it will lose effectiveness as the Ce6 will have to compete more with other compounds in the ocular pathways.
Any questions or flames, post 'em if you got 'em. -
2015-11-03 at 7 PM UTCLooking into this I was wondering why exactly it require insulin. From what I read there was no definitive reason as to using it.
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2015-11-04 at 5:33 AM UTC
Looking into this I was wondering why exactly it require insulin. From what I read there was no definitive reason as to using it.
Insulin does have a definite reason for its inclusion although it's not vital to achieve a difference in vision. It does, however, help achieve a bigger difference. The reason is that it effectively blocks ABCG2 from reaching photoreceptors in the eye allowing more Ce6 to bind with them instead. Ce6 will still bind but more receptors will be tied up with ABCG2 still. This is also part of why you have to wait for the Ce6 to "kick in", you're waiting for the ABCG2 to clear out somewhat from the photoreceptors and make way instead for the Ce6 which cannot bind until then.I was curious what other possible ill-effects it'd have on my body. And what visible differences there'd be to other people looking at me. Things like that.
In regards to these from the NO topic... First off there is no discernable difference in your physical appearance to others. In the SFM pics you see black eyes, but that's because he's (unnecessarily) wearing black sclera lenses to minimize light entering the eye. Of course, it wasn't known that it was unnecessary at the time because nobody knew what to expect. Dark sunglasses will get you by in lit areas but you still don't want to look at bright objects even with the sunglasses on.
The main known concern with ce6 is cellular necrosis but this is to my knowledge only able to happen when the eyeball is hit with a laser meant to specifically target Ce6-doped cells, heating them up and killing the cell. This is called PDT and is a cancer killing method. It would be very hard to bring this onto yourself accidentally - being hit with headlights or spotlights isn't enough and you wouldn't be able to not look away well before the possibility of damage.
Unfortunately, beyond that it's unknown. I've used this about 10 times now with no damage to my eyes - yet. The author from SFM who was the subject can also still see fine. Nobody is making you use Ce6 - if you do, you are a test subject. There is still a lot of unknowns.
The one effect I can say is that after you come back in, being in lights even with shades on can be headache inducing. I've gone to bed early during a few test nights. -
2015-11-04 at 3:20 PM UTCand follow soe cases or read in the news the type sof charges people get like this guy who attacked a veteranarian and his own dog also attacked and bit her:https://tvn24.pl/tvnwarszawa/najnowsze/otwock-lekarka-nie-chciala-uspic-psa-wlasciciel-rzucil-sie-na-nia-z-piesciami-6784005it seems nothing like US of A or the so called land of the free and the brave. Like they dont seem to pile charges on and also you wont be charged I dont think with murde ror accessory to it in poland just for being in a car full of gang memebrs or knuckle heads like you do cosntantly in california hear about or simply because you robbed some guy and he decided to take a gun out and your friend shot him or something.
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2015-11-04 at 3:22 PM UTCAlso, this is just an idea I had. Seeing as you use saline solution, I wear contacts already. Could I theoretically just put the Ce6 solution into my contact lens storage cases and soak an extra pair for Night Op use?
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2015-11-05 at 3:48 AM UTCThat seems like a pretty bad idea to be honest. For one, I suspect it would wear on the contacts quickly and degrade them. Even if it didn't, I don't think enough solution would come up with the contacts and into the eye as needed. It's better to just take the contacts out, use the drops, and then immediately replace the contacts back into your eyes. I myself wear contacts and haven't had any troubles using this method. It's also worth mentioning that if one tried to soak the contacts, you'd need to keep them in the fridge to keep them cool enough as to prevent your Ce6 and insulin from degrading.
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2015-11-05 at 11:25 AM UTCwhat the fuck is going on here? can you give me a tldr?
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2015-11-05 at 7:10 PM UTC
That seems like a pretty bad idea to be honest. For one, I suspect it would wear on the contacts quickly and degrade them. Even if it didn't, I don't think enough solution would come up with the contacts and into the eye as needed. It's better to just take the contacts out, use the drops, and then immediately replace the contacts back into your eyes. I myself wear contacts and haven't had any troubles using this method. It's also worth mentioning that if one tried to soak the contacts, you'd need to keep them in the fridge to keep them cool enough as to prevent your Ce6 and insulin from degrading.
The fridge thing actually came to mind afterwards and I decided it was to much work. -
2015-11-05 at 8:24 PM UTCYou're going to go blind from this.
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2015-11-05 at 11:49 PM UTC
You're going to go blind from this.
Alright, thanks for the heads up.what the fuck is going on here? can you give me a tldr?
A form of night vision drops, basically. NV via light amplification at night, no spectrum shift. Spectrum shifts will be discussed in future experiments. -
2015-11-17 at 4:10 PM UTCI finally have acquired the insulin. Got it for free from my friend, a diabetic. My only concern is whether this type will work because he's told me insulin burns like hell if it gets in an open wound, so my thoughts are, what the fuck would it do if in my eyes. It's a 100mL vial with approx 50mL left. They cost him about $600 a vial but with medical insurance he pays like $100, he also has a supply of needles if need be for measuring it out.
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2015-11-18 at 3:25 PM UTCKeep in mind that the insulin will be quite diluted in the solution, the drops should NOT sting upon contact. They are mildly irritating but that's also considering you have to keep your eye from blinking the solution out while you wait for them to absorb. They shouldn't burn, itch, or otherwise feel like anything other than cool liquid on your eyeball when administering.
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2015-11-23 at 4:01 PM UTC
Keep in mind that the insulin will be quite diluted in the solution, the drops should NOT sting upon contact. They are mildly irritating but that's also considering you have to keep your eye from blinking the solution out while you wait for them to absorb. They shouldn't burn, itch, or otherwise feel like anything other than cool liquid on your eyeball when administering.
The brand won't matter, correct? -
2015-11-24 at 2:51 AM UTCThere can be a bit of a difference. Any insulin will work but the dynamics are different between different types. Generally insulins are categorized by onset, peak time and total duration. Some are also categorized by how they're meant to be injected but that's not important for this. The vast majority of insulins are simply regular human insulin. More information here:
http://dtc[dot]ucsf.edu/types-of-diabetes/type2/treatment-of-type-2-diabetes/medications-and-therapies/type-2-insulin-rx/types-of-insulin/
TL/DR version: If there's no mention of intermediate acting or long acting, you're fine. And while it may seem tempting to have long lasting effects, you have to remember that the Ce6 will completely cycle through anyway and any absorbed insulin left will not be helpful at all.
